Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-DPP4 antibody [EPR21945] (ab222716)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21945] to DPP4
Suitable for: ELISA, Flow Cyt, ICC/IF, IP, WB
Reacts with: Mouse, Recombinant fragment

Product name

Anti-DPP4 antibody [EPR21945]
See all DPP4 primary antibodies
Description

Rabbit monoclonal [EPR21945] to DPP4
Host species

Rabbit

Tested Applications & Species

Application	Species
ELISA	Recombinant fragment
Flow Cyt	Mouse
ICC/IF	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse lung and liver tissue lysates. ICC/IF: Mouse peripheral blood mononuclear cells. Flow cyt: Mouse peripheral blood mononuclear cells. IP: Mouse lung tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-DPP4 antibody [EPR21945] (ab222716)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse PBMC (mouse peripheral blood mononuclear cells) cells labeling CD26 with ab222716 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in mouse PBMCs. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Western blot - Anti-DPP4 antibody [EPR21945] (ab222716)

All lanes : Anti-DPP4 antibody [EPR21945] (ab222716) at 1/1000 dilution

Lane 1 : Mouse lung tissue lysate
Lane 2 : Mouse liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 87 kDa
Observed band size: 110 kDa
why is the actual band size different from the predicted?

Blocking/Dilution: 5% NFDM/TBST

Exposure.

Lane 1: 147 seconds, Lane 2: 37 seconds
Flow Cytometry - Anti-DPP4 antibody [EPR21945] (ab222716)

Flow cytometric analysis of mouse PBMCs (mouse peripheral blood mononuclear cells) labeling CD26 with ab222716 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.
Immunoprecipitation - Anti-DPP4 antibody [EPR21945] (ab222716)

CD26 was immunoprecipitated from 0.35mg of mouse lung tissue lysate with ab222716 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222716 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: Mouse lung tissue lysate 10 μg (Input).

Lane 2: ab222716 IP in mouse lung tissue lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222716 in mouse lung tissue lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
ELISA - Anti-DPP4 antibody [EPR21945] (ab222716)

ELISA analysis of Mouse Dpp4 recombinant protein at 1000 ng/mL with ab222716. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
Anti-DPP4 antibody [EPR21945] (ab222716)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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