Anti-Histone H3 (phospho S28) antibody (ab183767)
Key features and details
- Rabbit polyclonal to Histone H3 (phospho S28)
- Suitable for: WB, Dot blot, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (phospho S28) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (phospho S28) -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blot, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chimpanzee
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Immunogen
Synthetic peptide corresponding to Human Histone H3 (phospho S28).
Database link: P68431 -
Positive control
- HeLa whole cell lysate (100 ng/ml Nocodazole treatment for 24 hr); Human breast carcinoma and gastric carcinoma tissues; HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3 (phospho S28) antibody (ab183767)All lanes : Anti-Histone H3 (phospho S28) antibody (ab183767) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate (untreated)
Lane 2 : HeLa whole cell lysate (100 ng/ml Nocodazole treatment for 24 hr)
Lysates/proteins at 30 µg per lane.
Predicted band size: 15 kDa15% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho S28) antibody (ab183767)Immunofluorescent analysis of asynchronous HeLa cells (fixed in 2% paraformaldehyde for 30 mins) labeling Histone H3 (phospho S28) at 1/500 dilution (green). Counter stained with Hoechst 33342 (blue) in bottom panels.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S28) antibody (ab183767)Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling Histone H3 (phospho S28) with ab183767 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S28) antibody (ab183767)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Histone H3 (phospho S28) with ab183767 at 1/500 dilution.
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Dot Blot - Anti-Histone H3 (phospho S28) antibody (ab183767)Dot blot analysis labeling Histone H3 (phospho S28).
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with ab183767 at 1/5000 dilution.
1: Peptide samples of H3S10 un
2: Peptide samples of H3S10ph
3: Peptide samples of H3S28 un
4: Peptide samples of H3S28ph
