Anti-Histone H3 (phospho S28) antibody [EP1874Y] (ab76312)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1874Y] to Histone H3 (phospho S28)
- Suitable for: WB, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-Histone H3 (phospho S28) antibody [EP1874Y]
See all Histone H3 primary antibodies -
Description
Rabbit monoclonal [EP1874Y] to Histone H3 (phospho S28) -
Host species
Rabbit -
Specificity
This antibody detects Histone H3 and Histone H3.3 when phosphorylated on Serine 28. It does not detect H3.3 when phosphorylated on Serine 31. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Histone H3 aa 1-100 (phospho S28).
Database link: P84243 -
Positive control
- HeLa cell lysates treated with FBS + calyculin A.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1874Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Histone H3 (phospho S28) antibody [EP1874Y] (ab76312) at 1/5000 dilution
Lane 1 : HeLa cell lysates untreated
Lane 2 : HeLa cell lysates treated with FBS + calyculin A
Lysates/proteins at 10 µg per lane.
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
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Dot blot performed using ab76312 at a dilution of 1/100. Lane 1 - Unmodified H3 peptide. Lane 2 - H3S28ph peptide. Lane 3 - H3.3S28ph peptide. Lane 4 - H3.3S31ph peptide. A HRP-conjugated goat anti-rabbit (H+L) was used as the secondary antibody at a dilution of 1/1000. The exposure time was 3 minutes and the dilution and blocking buffer used were 5% NFDM/TBST.
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