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Epigenetics and Nuclear Signaling Histones Variants

Anti-Histone H3 (phospho S28) antibody [EP1874Y] (ab76312)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-Histone H3 (phospho S28) antibody [EP1874Y] (ab76312)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1874Y] to Histone H3 (phospho S28)
  • Suitable for: WB, Dot blot
  • Reacts with: Human

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Overview

  • Product name

    Anti-Histone H3 (phospho S28) antibody [EP1874Y]
    See all Histone H3 primary antibodies
  • Description

    Rabbit monoclonal [EP1874Y] to Histone H3 (phospho S28)
  • Host species

    Rabbit
  • Specificity

    This antibody detects Histone H3 and Histone H3.3 when phosphorylated on Serine 28. It does not detect H3.3 when phosphorylated on Serine 31.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Histone H3 aa 1-100 (phospho S28).
    Database link: P84243

  • Positive control

    • HeLa cell lysates treated with FBS + calyculin A.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP1874Y
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • Variants
    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Phosphorylated

Images

  • Western blot - Anti-Histone H3 (phospho S28) antibody [EP1874Y] (ab76312)
    Western blot - Anti-Histone H3 (phospho S28) antibody [EP1874Y] (ab76312)
    All lanes : Anti-Histone H3 (phospho S28) antibody [EP1874Y] (ab76312) at 1/5000 dilution

    Lane 1 : HeLa cell lysates untreated
    Lane 2 : HeLa cell lysates treated with FBS + calyculin A

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 15 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?

  • Dot Blot - Anti-Histone H3 (phospho S28) antibody [EP1874Y] (ab76312)
    Dot Blot - Anti-Histone H3 (phospho S28) antibody [EP1874Y] (ab76312)

    Dot blot performed using ab76312 at a dilution of 1/100. Lane 1 - Unmodified H3 peptide. Lane 2 - H3S28ph peptide. Lane 3 - H3.3S28ph peptide. Lane 4 - H3.3S31ph peptide. A HRP-conjugated goat anti-rabbit (H+L) was used as the secondary antibody at a dilution of 1/1000. The exposure time was 3 minutes and the dilution and blocking buffer used were 5% NFDM/TBST.

  • Anti-Histone H3 (phospho S28) antibody [EP1874Y] (ab76312)
    Anti-Histone H3 (phospho S28) antibody [EP1874Y] (ab76312)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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