Anti-Histone H2A.Z antibody [4A4] (ab80150)
![Anti-Histone H2A.Z antibody [4A4] (ab80150)](https://www.abcam.com/ps/products/80/ab80150/Images/ab80150-95482-anti-histone-h2az-antibody-4a4-western-blot.jpg "Anti-Histone H2A.Z antibody [4A4] (ab80150)")
Key features and details
- Rat monoclonal [4A4] to Histone H2A.Z
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-Histone H2A.Z antibody [4A4]
See all Histone H2A.Z primary antibodies -
Description
Rat monoclonal [4A4] to Histone H2A.Z -
Host species
Rat -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Mouse, Rat, Cow, Human, Pig, Xenopus laevis, Zebrafish, Orangutan
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Immunogen
Synthetic peptide corresponding to Human Histone H2A.Z.
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Positive control
- This antibody gave a positive signal when used to probe Histone H2A.Z recombinant protein.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
4A4 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-Histone H2A.Z antibody [4A4] (ab80150)All lanes : Anti-Histone H2A.Z antibody [4A4] (ab80150) at 1 µg/ml
Lane 1 : Histone H2A.Z recombinant protein
Lane 2 : Histone H2A recombinant protein
Lysates/proteins at 0.5 µg per lane.
Secondary
All lanes : Goat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
Predicted band size: 13 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
