Anti-Histone H2A.Z antibody [EPR6172] (ab124793)
![Anti-Histone H2A.Z antibody [EPR6172] (ab124793)](https://www.abcam.com/ps/products/124/ab124793/Images/ab124793-138681-anti-histone-h2az-antibody-epr6172-western-blot.jpg "Anti-Histone H2A.Z antibody [EPR6172] (ab124793)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6172] to Histone H2A.Z
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Histone H2A.Z antibody [EPR6172]
See all Histone H2A.Z primary antibodies -
Description
Rabbit monoclonal [EPR6172] to Histone H2A.Z -
Host species
Rabbit -
Specificity
This antibody is known to detect both isoforms of H2A.Z (H2A.Z.1 and H2A.Z.2). -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Rat
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Immunogen
Synthetic peptide within Human Histone H2A.Z aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
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Positive control
- C6, Raw 264.7, PC-12, NIH/3T3, HeLa, HepG2, and Human thymus lysates , Human breast tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6172 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H2A.Z antibody [EPR6172] (ab124793)All lanes : Anti-Histone H2A.Z antibody [EPR6172] (ab124793) at 1/1000 dilution
Lane 1 : C6 lysates
Lane 2 : Raw 264.7 lysates
Lane 3 : PC-12 lysates
Lane 4 : NIH/3T3 lysates
Lane 5 : HeLa lysates
Lane 6 : HepG2 lysates
Lane 7 : Human thymus lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 14 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6172] (ab124793)](https://www.abcam.com/ps/products/124/ab124793/Images/ab124793-138680-anti-histone-h2az-antibody-epr6172-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6172] (ab124793)ab124793, at a 1/250 dilution, staining Histone H2A.Z in paraffin embedded Human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Histone H2A.Z antibody [EPR6172] (ab124793)](https://www.abcam.com/ps/products/124/ab124793/Images/ab124793-3-benefits-of-recombinant-antibodies.png)
Anti-Histone H2A.Z antibody [EPR6172] (ab124793)
