Antibodies, Proteins, Kits and Reagents for Life Science

334 369 ₸ Product size

100 µl 334 369 ₸ 100 µl 334 369 ₸

Order now and get it on Tuesday March 09, 2021

HRP Anti-mH2A1 antibody [EPR9359(2)] (ab209320)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
HRP Rabbit monoclonal [EPR9359(2)] to mH2A1
Suitable for: WB
Knockout validated
Reacts with: Human
Conjugation: HRP

Product name

HRP Anti-mH2A1 antibody [EPR9359(2)]
See all mH2A1 primary antibodies
Description

HRP Rabbit monoclonal [EPR9359(2)] to mH2A1
Host species

Rabbit
Conjugation

HRP
Tested Applications & Species

Application Species
ICC
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HepG2, HeLa and HEK293 whole cell lysate.
General notes
Previously labelled as macroH2A.1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9359(2)
Isotype

IgG
Research areas

Western blot - HRP Anti-mH2A1 antibody [EPR9359(2)] (ab209320)

All lanes : HRP Anti-mH2A1 antibody [EPR9359(2)] (ab209320) at 1/5000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : H2AFY (macroH2A.1) knockout HAP1 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 40 kDa
Observed band size: 40 kDa

Exposure time: 90 seconds

ab209320 was shown to recognize macroH2A.1 in wild-type HAP1 cells as signal was lost at the expected MW in H2AFY (macroH2A.1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and H2AFY (macroH2A.1) knockout samples were subjected to SDS-PAGE. Ab209320 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor^® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
Western blot - HRP Anti-mH2A1 antibody [EPR9359(2)] (ab209320)

All lanes : HRP Anti-mH2A1 antibody [EPR9359(2)] (ab209320) at 1/5000 dilution

Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa whole cell lysate (ab150035)
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 40 kDa
Observed band size: 40 kDa

Exposure time: 12 minutes

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209320 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
HRP Anti-mH2A1 antibody [EPR9359(2)] (ab209320)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com