Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6861] to CD79b - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CD79b antibody [EPR6861] - BSA and Azide free
    See all CD79b primary antibodies

  • Description

    Rabbit monoclonal [EPR6861] to CD79b - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: Daudi cells; IHC-P: Human tonsil, Mouse and Rat spleen tissue; Flow Cyt: Raji cells. WB: Raji whole lysate. Mouse and rat spleen lysates.

  • General notes

    ab240083 is the carrier-free version of ab134147.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Dissociation constant (KD)

    KD = 4.70 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR6861

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)
    Immunocytochemistry/ Immunofluorescence - Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)

    Immunofluorescence staining of Daudi cells with purified ab134147 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134147).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)

    Immunohistochemical analysis of paraffin embedded Human spleen tissue labelled with ab134147 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134147).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)
    Flow Cytometry - Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)

    Flow Cytometry analysis of Raji cells labeling CD79b with ab134147 at 1/20 dilution (red).  A rabbit IgG was used as negative control (green). 

    This data was produced with ab134147, the same antibody in a diffrent forulation with BSA and Azide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)

    Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelled with ab134147 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134147).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)

    ab134147 showing positive staining in Diffuse B-cell lymphoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134147).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)

    ab134147 showing negative staining in Normal kidney tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134147).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)

    ab134147 showing negative staining in Skeletal muscle tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134147).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)

    ab134147 showing negative staining in Normal brain tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134147).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)
    OI-RD Scanning - Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134147).

  • Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)
    Anti-CD79b antibody [EPR6861] - BSA and Azide free (ab240083)

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