Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸ 10 µl 127 315 ₸

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Anti-CD79b antibody [EPR6861] (ab134147)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6861] to CD79b
Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-CD79b antibody [EPR6861]
See all CD79b primary antibodies
Description

Rabbit monoclonal [EPR6861] to CD79b
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to residues on the C-terminal in Human CD79b (P40259).
Positive control
- Daudi, Namalwa, and Raji cell lysates, Human spleen and tonsil Tissue Flow Cyt: Raji cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Dissociation constant (K_D)

K_D = 4.70 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR6861
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)

Immunohistochemical analysis of paraffin embedded Human spleen tissue labelled with ab134147 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-CD79b antibody [EPR6861] (ab134147)

All lanes : Anti-CD79b antibody [EPR6861] (ab134147) at 1/1000 dilution

Lane 1 : Daudi cell lysates
Lane 2 : Namalwa cell lysates
Lane 3 : Raji cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 26 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CD79b antibody [EPR6861] (ab134147)

Immunofluorescence staining of Daudi cells with purified ab134147 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor^®488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.
Flow Cytometry - Anti-CD79b antibody [EPR6861] (ab134147)

Flow Cytometry analysis of Raji cells labeling CD79b with ab134147 at 1/20 dilution (red). A rabbit IgG was used as negative control (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelled with ab134147 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)

ab134147 showing positive staining in Diffuse B-cell lymphoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)

ab134147 showing negative staining in Normal kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)

ab134147 showing negative staining in Skeletal muscle tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)

ab134147 showing negative staining in Normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
OI-RD Scanning - Anti-CD79b antibody [EPR6861] (ab134147)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-CD79b antibody [EPR6861] (ab134147)

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