Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD79b antibody [EPR6861] (ab134147)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6861] to CD79b
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD79b antibody [EPR6861]
    See all CD79b primary antibodies

  • Description

    Rabbit monoclonal [EPR6861] to CD79b

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues on the C-terminal in Human CD79b (P40259).

  • Positive control

    • Daudi, Namalwa, and Raji cell lysates, Human spleen and tonsil Tissue Flow Cyt: Raji cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Dissociation constant (KD)

    KD = 4.70 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR6861

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)

    Immunohistochemical analysis of paraffin embedded Human spleen tissue labelled with ab134147 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-CD79b antibody [EPR6861] (ab134147)
    Western blot - Anti-CD79b antibody [EPR6861] (ab134147)
    All lanes : Anti-CD79b antibody [EPR6861] (ab134147) at 1/1000 dilution

    Lane 1 : Daudi cell lysates
    Lane 2 : Namalwa cell lysates
    Lane 3 : Raji cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 26 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CD79b antibody [EPR6861] (ab134147)
    Immunocytochemistry/ Immunofluorescence - Anti-CD79b antibody [EPR6861] (ab134147)

    Immunofluorescence staining of Daudi cells with purified ab134147 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

  • Flow Cytometry - Anti-CD79b antibody [EPR6861] (ab134147)
    Flow Cytometry - Anti-CD79b antibody [EPR6861] (ab134147)

    Flow Cytometry analysis of Raji cells labeling CD79b with ab134147 at 1/20 dilution (red).  A rabbit IgG was used as negative control (green). 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)

    Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelled with ab134147 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)

    ab134147 showing positive staining in Diffuse B-cell lymphoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)

    ab134147 showing negative staining in Normal kidney tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)

    ab134147 showing negative staining in Skeletal muscle tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)

    ab134147 showing negative staining in Normal brain tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-CD79b antibody [EPR6861] (ab134147)
    OI-RD Scanning - Anti-CD79b antibody [EPR6861] (ab134147)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CD79b antibody [EPR6861] (ab134147)
    Anti-CD79b antibody [EPR6861] (ab134147)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD79b antibody [EPR6861] (ab134147)

© 2021 Astana Biomed Group LLP. All rights reserved.