Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD19 antibody [SP291] - C-terminal (ab227688)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP291] to CD19 - C-terminal
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CD19 antibody [SP291] - C-terminal
    See all CD19 primary antibodies

  • Description

    Rabbit monoclonal [SP291] to CD19 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Ramos cell lysate. Flow Cyt: Ramos and Raji cells. IHC-P: Human tonsil, spleen, colon, thymus, B-Cell lymphoma and breast ductal carcinoma tissues.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD19 with ab227688 at 1/100 dilution (1.50 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227688 for 10 mins at room temperature.

  • Flow Cytometry - Anti-CD19 antibody [SP291] - C-terminal (ab227688)
    Flow Cytometry - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Flow cytometry analysis of Raji (human Burkitt's lymphoma) labeling CD19 with purified ab227688 at 1/20 dilution (7.5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

  • Western blot - Anti-CD19 antibody [SP291] - C-terminal (ab227688)
    Western blot - Anti-CD19 antibody [SP291] - C-terminal (ab227688)
    Anti-CD19 antibody [SP291] - C-terminal (ab227688) at 1/400 dilution + Ramos (human Burkitt's lymphoma cell line) cell lysate

    Predicted band size: 61 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for CD19 using ab227688 at 1/100 dilution in immunohistochemical analysis.

  • Flow Cytometry - Anti-CD19 antibody [SP291] - C-terminal (ab227688)
    Flow Cytometry - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Flow Cytometry analysis of Ramos (human Burkitt's lymphoma cell line) cells, labeling CD19 with ab227688 at 1/400 dilution (green) compared to a Rabbit IgG negative control (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Formalin-fixed, paraffin-embedded human colon tissue stained for CD19 using ab227688 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Formalin-fixed, paraffin-embedded human spleen tissue stained for CD19 using ab227688 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Formalin-fixed, paraffin-embedded human thymus tissue stained for CD19 using ab227688 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Formalin-fixed, paraffin-embedded human B-Cell lymphoma tissue stained for CD19 using ab227688 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD19 using ab227688 at 1/100 dilution in immunohistochemical analysis.

  • Anti-CD19 antibody [SP291] - C-terminal (ab227688)
    Anti-CD19 antibody [SP291] - C-terminal (ab227688)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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