Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸ 10 µl 137 366 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5906] to CD19
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-CD19 antibody [EPR5906]
See all CD19 primary antibodies
Description

Rabbit monoclonal [EPR5906] to CD19
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment within Human CD19 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: P15391
Positive control
- WB: Namalwa, Daudi and Ramos cell lysates; human tonsil tissue lysate. IHC-P: Human tonsil, diffuse large B-cell lymphoma, B-cell chronic lymphocytic leukaemia and spleen tissue. ICC/IF: Raji cells. Flow Cyt: Raji cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR5906
Isotype

IgG
Research areas

Flow Cytometry - Anti-CD19 antibody [EPR5906] (ab134114)

Flow cytometry analysis of Raji cells (Human Burkitt's lymphoma B lymphocyte) labelling CD19 with ab134114 at 1:1000 dilution, 1.186 µg/ml (red). Cells were fixed with 4% paraformaldehyde, permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000.

Isotype control (black) - Rabbit monoclonal IgG (ab172730)

Unlabeled control (blue) - Unlabelled cells
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR5906] (ab134114)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD19 with unpurified ab134114 at a dilution of 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-CD19 antibody [EPR5906] (ab134114)

Immunocytochemistry/Immunofluorescence analysis of Raji cells labelling CD19 with purified ab134114 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).

Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).
Western blot - Anti-CD19 antibody [EPR5906] (ab134114)

Lanes 1-3 : Anti-CD19 antibody [EPR5906] (ab134114) at 1/10000 dilution
Lane 4 : Anti-CD19 antibody [EPR5906] (ab134114) at 1/2000 dilution

Lane 1 : NAMALWA (Human Burkitt's lymphoma) whole cell lysate)
Lane 2 : Human tonsil tissue lysate
Lane 3 : Jurkat (Human acute T cell leukemia)whole cell lysate
Lane 4 : RAMOS (Human Burkitt's lymphoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 95 kDa
why is the actual band size different from the predicted?

Exposure time:
Lane 1-3: 3 minutes
Lane 4: 30 seconds

Jurkat is a CD19 null cell line according to the paper (PMID: 19147785).
Western blot - Anti-CD19 antibody [EPR5906] (ab134114)

All lanes : Anti-CD19 antibody [EPR5906] (ab134114) at 1/5000 dilution (purified)

Lane 1 : Namalwa whole cell lysate
Lane 2 : Daudi whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 61 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR5906] (ab134114)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD19 with purified ab134114 at a dilution of 1/500. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR5906] (ab134114)

Negative tissue: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling CD19 with purified ab134114 at a dilution of 1/500. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR5906] (ab134114)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human diffuse large B-cell lymphoma tissue labelling CD19 with unpurified ab134114.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR5906] (ab134114)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CD19 with unpurified ab134114.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR5906] (ab134114)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human B-cell chronic lymphocytic leukaemia tissue labelling CD19 with unpurified ab134114.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR5906] (ab134114)

Immunohistochemical analysis of paraffin embedded human skeletal muscle tissue using unpurified ab134114 showing negative staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-CD19 antibody [EPR5906] (ab134114)

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