Anti-CD19 antibody (ab227019)
Key features and details
- Rabbit polyclonal to CD19
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CD19 antibody
See all CD19 primary antibodies -
Description
Rabbit polyclonal to CD19 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human CD19 (internal sequence). The exact sequence is proprietary.
Database link: P15391 -
Positive control
- Flow Cyt: Mouse splenocytes. WB: Mouse leukocyte extract; Raji whole cell lysate. ICC/IF: Raji cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Flow cytometric analysis of 4% paraformaldehyde-fixed mouse splenocytes labeling CD19 with ab227019 at 1/50 dilution (red) compared with an unlabeled sample as a control (black).
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
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Anti-CD19 antibody (ab227019) at 1/1000 dilution + Mouse leukocyte extract at 50 µg
Predicted band size: 61 kDa7.5% SDS-PAGE gel.
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Raji (human Burkitt's lymphoma cell line) cells stained for CD19 (green) using ab227019 at 1/1000 dilution in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes.
Blue: Hoechst 33342 staining.
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Anti-CD19 antibody (ab227019) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Predicted band size: 61 kDa7.5 % SDS-PAGE gel.