Blocking - Blocking peptide for Anti-Myc tag antibody [9E10] (ab32), Anti-Myc tag antibody (ab172), Agarose Anti-Myc tag antibody (ab1253), HRP Anti-Myc tag antibody (ab1261), FITC Anti-Myc tag antibody (ab1263), HRP Anti-Myc tag antibody (ab1326), FITC Anti-Myc tag antibody (ab1393), FITC Anti-Myc tag antibody (ab1394), Anti-Myc tag antibody (ab9106), Anti-Myc tag antibody (ab9109), Anti-Myc tag antibody - ChIP Grade (ab9132), Anti-Myc tag antibody [Myc.A7] (ab18185)

- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.