Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1216] to SDF1 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-SDF1 antibody [EPR1216] - BSA and Azide free
    See all SDF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR1216] to SDF1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Mouse cross reactivity based on sequence analysis only. 100% sequence homology for isforms 1-6 (P48061; isoforms 1-6).

  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human SDF1 alpha recombinant protein; Jurkat cells.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    ab271917 is the carrier-free version of ab155090. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)
    Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)

    Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia cell line) labeling SDF1 with Purified ab155090 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155090).
  • Flow Cytometry - Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)
    Flow Cytometry - Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)

    Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling SDF1 with purified ab155090 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155090).
  • Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)
    Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)

    Immunofluorescent analysis of Jurkat cells, labeling SDF1 with ab155090 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155090).
  • Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)
    Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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