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Immunology Innate Immunity Chemokines Alpha Chemokines (CXC)

Anti-SDF1 antibody [EPR1216] (ab155090)

Price and availability

314 937 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-SDF1 antibody [EPR1216] (ab155090)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1216] to SDF1
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-SDF1 antibody [EPR1216]
    See all SDF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR1216] to SDF1
  • Host species

    Rabbit
  • Specificity

    Mouse cross reactivity based on sequence analysis only. 100% sequence homology for isforms 1-6 (P48061; isoforms 1-6).
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human SDF1 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: P48061

  • Positive control

    • Human SDF1 alpha recombinant protein; Jurkat cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR1216
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokines (CXC)
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Chemokines

Images

  • Western blot - Anti-SDF1 antibody [EPR1216] (ab155090)
    Western blot - Anti-SDF1 antibody [EPR1216] (ab155090)
    Anti-SDF1 antibody [EPR1216] (ab155090) at 1/10000 dilution + Human CXCL12 recombinant protein at 0.01 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 11 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (ab155090)
    Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (ab155090)

    Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia cell line) labeling SDF1 with Purified ab155090 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Flow Cytometry - Anti-SDF1 antibody [EPR1216] (ab155090)
    Flow Cytometry - Anti-SDF1 antibody [EPR1216] (ab155090)

    Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling SDF1 with purified ab155090 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

  • Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (ab155090)
    Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (ab155090)

    Immunofluorescent analysis of Jurkat cells, labeling SDF1 with ab155090 at 1/100 dilution.

  • Anti-SDF1 antibody [EPR1216] (ab155090)
    Anti-SDF1 antibody [EPR1216] (ab155090)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-SDF1 antibody [EPR1216] (ab155090)

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    Anti-SDF1 antibody [EPR1216] - BSA and Azide free (ab271917)

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