Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SDF1 antibody [EPR1216] - Low endotoxin, Azide free (ab157772)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1216] to SDF1 - Low endotoxin, Azide free
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-SDF1 antibody [EPR1216] - Low endotoxin, Azide free
    See all SDF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR1216] to SDF1 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: MouseDoes not react with: Rat

  • Immunogen

    Synthetic peptide corresponding to SDF1.
    Database link: P48061

  • General notes

    ab157772 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-SDF1 antibody [EPR1216] - Low endotoxin, Azide free (ab157772)
    Flow Cytometry - Anti-SDF1 antibody [EPR1216] - Low endotoxin, Azide free (ab157772)

    Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling SDF1 with purified ab155090 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155090).

  • Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] - Low endotoxin, Azide free (ab157772)
    Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] - Low endotoxin, Azide free (ab157772)

    This ICC data was generated using the same anti-SDF1 antibody clone, EPR1216, in a different buffer formulation (cat# ab155090).

    Immunofluorescent analysis of Jurkat cells, labeling SDF1 with ab155090 at 1/100 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] - Low endotoxin, Azide free (ab157772)
    Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] - Low endotoxin, Azide free (ab157772)

    This ICC data was generated using the same anti-SDF1 antibody clone, EPR1216, in a different buffer formulation (cat# ab155090).

    Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia cell line) labeling SDF1 with Purified ab155090 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Anti-SDF1 antibody [EPR1216] - Low endotoxin, Azide free (ab157772)
    Anti-SDF1 antibody [EPR1216] - Low endotoxin, Azide free (ab157772)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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