Anti-SDF1 antibody (ab18919)
Key features and details
- Rabbit polyclonal to SDF1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SDF1 antibody
See all SDF1 primary antibodies -
Description
Rabbit polyclonal to SDF1 -
Host species
Rabbit -
Specificity
The antibody should cross-react to both P40224-1 and P40224-2. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Mouse SDF1 aa 1-93. E. coli-expressed mouse SDF1.
Database link: P40224
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SDF1 antibody (ab18919)
Western blot analysis of SDF-1? expression in transfected cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (ab18919)ab18919 (2µg/ml) staining SDF1 in human gallbladder using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic/secretory compartments within the smooth muscle fibres.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
