Anti-SDF1 antibody (ab9797)
Key features and details
- Rabbit polyclonal to SDF1
- Suitable for: Sandwich ELISA, WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: unknown
Overview
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Product name
Anti-SDF1 antibody
See all SDF1 primary antibodies -
Description
Rabbit polyclonal to SDF1 -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISA, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Goat
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Immunogen
Recombinant fragment corresponding to Human SDF1 aa 22-89.
Sequence:KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK
Database link: P48061 -
Positive control
- IHC-P: Human lung cancer and human kidney tissues; WB: Recombinant human SDF1 protein (ab9798).
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General notes
Although some customers have been successful in IHC we no longer batch test in this application.
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (ab9797)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SDF1 with ab9797 at 2 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (ab9797)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling SDF1 with ab9797 at 2 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
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Sandwich ELISA - Anti-SDF1 antibody (ab9797)ELISA - Anti-IL-1 beta antibody (ab9797)Sandwich ELISA detecting SDF1 using ab9797 at a concentration of 2.0 µg/ml.
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Western blot - Anti-SDF1 antibody (ab9797)To detect human SDSF1 by Western Blot analysis, ab9739 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant human SDF1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human SDF1, respectively.
Non-reducing conditions.
