Recombinant human c-Myc protein (Active) (ab169901)
Key features and details
- Expression system: Escherichia coli
- Purity: > 93% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE, MS
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Product name
Recombinant human c-Myc protein (Active)
See all c-Myc proteins and peptides -
Biological activity
Reprogramming mouse fibroblast cell to iPS cells using 3 retroviral vectors, which carry Oct4, Sox2 & Klf4 with this protein as replacement assay. 8 μg/ml of human Klf4-11R were added in reprogramming medium every 48 hours for 20 days.
Intracellular protein penetration rate was tested using DyLight labeled ab169901 protein at 1 μg/ ml for 30 min incubation for human fibroblast cells at 37oC. More than 90% cell will be positive one hour after sample incubation.
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Purity
> 93 % SDS-PAGE.
ab169901 was expressed in E. coli as inclusion bodies, solubilized, refolded, and further purified. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MDFFRVVENQ QPPATMPLNV SFTNRNYDLD YDSVQPYFYC DEEENFYQQQ QQSELQPPAP SEDIWKKFEL LPTPPLSPSR RSGLCSPSYV AVTPFSLRGD NDGGGGSFST ADQLEMVTEL LGGDMVNQSF ICDPDDETFI KNIIIQDCMW SGFSAAAKLV SEKLASYQAA RKDSGSPNPA RGHSVCSTSS LYLQDLSAAA SECIDPSVVF PYPLNDSSSP KSCASQDSSA FSPSSDSLLS STESSPQGSP EPLVLHEETP PTTSSDSEEE QEDEEEIDVV SVEKRQAPGK RSESGSPSAG GHSKPPHSPL VLKRCHVSTH QHNYAAPPST RKDYPAAKRV KLDSVRVLRQ ISNNRKCTSP RSSDTEENVK RRTHNVLERQ RRNELKRSFF ALRDQIPELE NNEKAPKVVI LKKATAYILS VQAEEQKLIS EEDLLRKRRE QLKHKLEQLR NSCAESGGGG SPGRRRRRRR RRRR -
Predicted molecular weight
53 kDa -
Amino acids
1 to 454 -
Additional sequence information
Please note that ab169901 is isoform 2 of UniProt accession P01106. (NP_002458.2)
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.50
Constituent: 0.24% Tris
Contains NaCl , KCl, CaCl2, MgCl2, arginine, DTT, and glycerol.This product is an active protein and may elicit a biological response in vivo, handle with caution.
