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Anti-Caspase-1 antibody [EPR19672] - BSA and Azide free (ab238979)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19672] to Caspase-1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Caspase-1 antibody [EPR19672] - BSA and Azide free
    See all Caspase-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19672] to Caspase-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human lymph node, thymus and spleen lysates.

  • General notes

    Ab238979 is the carrier-free version of ab207802. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238979 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-Caspase-1 antibody [EPR19672] - BSA and Azide free (ab238979)
    Immunoprecipitation - Anti-Caspase-1 antibody [EPR19672] - BSA and Azide free (ab238979)

    ab207802 Immunoprecipitating Caspase-1 in THP-1 (human acute monocytic leukemia) whole cell lysate. 2µg of capture antibody in 0.35mg lysate was used. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/1000 and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution .

    Lane 1: THP-1 (human acute monocytic leukemia ) whole cell lysate
    Lane 2: THP-1 (human acute monocytic leukemia ) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207802 in THP-1 (human acute monocytic leukemia ) whole cell lysate

    Blocking buffer: 5% NFDM/TBST

    Diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207802).

  • Western blot - Anti-Caspase-1 antibody [EPR19672] - BSA and Azide free (ab238979)
    Western blot - Anti-Caspase-1 antibody [EPR19672] - BSA and Azide free (ab238979)
    All lanes : Anti-Caspase-1 antibody [EPR19672] (ab207802) at 1/1000 dilution

    Lane 1 : Human lymph node lysate
    Lane 2 : Human thymus lysate
    Lane 3 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 45, 42, 35, 22, 20 kDa
    Observed band size: 22,35,45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Pro-Caspase 1 is cleaved into p20(p22) and p10(p12) subunits. PMID: 8662843. PMID: 7595215. The product recognizes p20(p22) cleavage fragment where the immunogen is located.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207802).

  • Anti-Caspase-1 antibody [EPR19672] - BSA and Azide free (ab238979)
    Anti-Caspase-1 antibody [EPR19672] - BSA and Azide free (ab238979)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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