Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-1 antibody [EPR19672] (ab207802)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19672] to Caspase-1
  • Suitable for: IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Caspase-1 antibody [EPR19672]
    See all Caspase-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19672] to Caspase-1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Untreated THP-1 whole cell lysate; THP-1 treated with 80 nM TPA overnight, then treated with 1 µg/ml LPS for 8 hours whole cell lysate; Human lymph node, thymus and spleen lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Caspase-1 antibody [EPR19672] (ab207802)
    Western blot - Anti-Caspase-1 antibody [EPR19672] (ab207802)
    All lanes : Anti-Caspase-1 antibody [EPR19672] (ab207802) at 1/1000 dilution

    Lane 1 : Untreated THP-1 (Human monocytic leukemia cell line) whole cell lysate
    Lane 2 : THP-1 (Human monocytic leukemia cell line) treated with 80 nM TPA overnight, then treated with 1 µg/ml LPS for 8 hours whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 45, 42, 35, 22, 20 kDa
    Observed band size: 20,22,42,45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    There are six pro-caspase 1 isoforms, generating products between 30-45kDa, Pro-Caspase 1 is cleaved into p20(p22) and p10(p12) subunits. PMID: 8662843. PMID: 7595215. The product recognizes p20(p22) cleavage fragment where the immunogen is located.

  • Western blot - Anti-Caspase-1 antibody [EPR19672] (ab207802)
    Western blot - Anti-Caspase-1 antibody [EPR19672] (ab207802)
    All lanes : Anti-Caspase-1 antibody [EPR19672] (ab207802) at 1/1000 dilution

    Lane 1 : Human lymph node lysate
    Lane 2 : Human thymus lysate
    Lane 3 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 45, 42, 35, 22, 20 kDa
    Observed band size: 22,35,45 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Pro-Caspase 1 is cleaved into p20(p22) and p10(p12) subunits. PMID: 8662843. PMID: 7595215. The product recognizes p20(p22) cleavage fragment where the immunogen is located.

  • Immunoprecipitation - Anti-Caspase-1 antibody [EPR19672] (ab207802)
    Immunoprecipitation - Anti-Caspase-1 antibody [EPR19672] (ab207802)

    ab207802 Immunoprecipitating Caspase-1 in THP-1 (human acute monocytic leukemia) whole cell lysate. 2µg of capture antibody in 0.35mg lysate was used. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/1000 and VeriBlot for IP Detection Reagent (HRP) ab131366 was used for detection at a dilution of 1/1000.

    Lane 1: THP-1 (human acute monocytic leukemia ) whole cell lysate
    Lane 2: THP-1 (human acute monocytic leukemia ) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207802 in THP-1 (human acute monocytic leukemia ) whole cell lysate

    Blocking buffer: 5% NFDM/TBST

    Diluting buffer: 5% NFDM/TBST

  • Anti-Caspase-1 antibody [EPR19672] (ab207802)
    Anti-Caspase-1 antibody [EPR19672] (ab207802)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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