Anti-Caspase-11 antibody [EPR18628] - BSA and Azide free (ab240991)
![Anti-Caspase-11 antibody [EPR18628] - BSA and Azide free (ab240991)](https://www.abcam.com/ps/products/240/ab240991/Images/ab240991-1-ab180673257647IP1.jpg "Anti-Caspase-11 antibody [EPR18628] - BSA and Azide free (ab240991)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18628] to Caspase-11 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse
Overview
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Product name
Anti-Caspase-11 antibody [EPR18628] - BSA and Azide free
See all Caspase-11 primary antibodies -
Description
Rabbit monoclonal [EPR18628] to Caspase-11 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: RAW 264.7 treated with Lipopolysaccharide 10µg/ml for 8 hours whole cell lysate.
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General notes
Ab240991 is the carrier-free version of ab180673. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240991 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18628 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Caspase-11 antibody [EPR18628] - BSA and Azide free (ab240991)
Caspase-11 was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate with ab180673 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab180673 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: RAW 264.7 treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate, 10µg (Input).
Lane 2: ab180673 IP in RAW 264.7 treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab180673 in RAW 264.7 treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180673).
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![Anti-Caspase-11 antibody [EPR18628] - BSA and Azide free (ab240991)](https://www.abcam.com/ps/products/240/ab240991/Images/ab240991-2-benefits-of-recombinant-antibodies.png)
Anti-Caspase-11 antibody [EPR18628] - BSA and Azide free (ab240991)
