Caspase-1 Assay Kit (Colorimetric) (ab273268)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Adherent cells, Cell Lysate, Suspension cells
Overview
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Product name
Caspase-1 Assay Kit (Colorimetric)
See all Caspase-1 kits -
Detection method
Colorimetric -
Sample type
Adherent cells, Suspension cells, Cell Lysate -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
Caspase-1 Assay Kit (Colorimetric) (ab273268) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate YVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 25 tests 2X Reaction Buffer 1 x 2ml Cell Lysis Buffer 1 x 25ml Dilution Buffer 1 x 25ml DTT 1 x 100µl YVAD-pNA (4 mM) 1 x 125µl -
Research areas
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Function
Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. -
Tissue specificity
Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
Post-translational
modificationsThe two subunits are derived from the precursor sequence by an autocatalytic mechanism. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- CASP-1
- CASP1
- CASP1_HUMAN
see all
Images
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Caspase-1 calibration curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Typical Caspase-1calibration curve using recombinant Caspase-1.
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Caspase-1 activity in uninduced and induced Jurkat cell lysatesCaspase-1 activity in uninduced and induced Jurkat cell lysates (1 µg).