HRP Anti-pro Caspase-3 antibody [E61] (ab205733)
![HRP Anti-pro Caspase-3 antibody [E61] (ab205733)](https://www.abcam.com/ps/products/205/ab205733/Images/ab205733-267750-IHCAP00234801.jpg "HRP Anti-pro Caspase-3 antibody [E61] (ab205733)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [E61] to pro Caspase-3
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-pro Caspase-3 antibody [E61]
See all pro Caspase-3 primary antibodies -
Description
HRP Rabbit monoclonal [E61] to pro Caspase-3 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and wildtype HAP1 (untreated and Stuarosporine treated) whole cell lysates. IHC-P: normal human tonsil tissue sections
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E61 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-pro Caspase-3 antibody [E61] (ab205733)
IHC image of pro Caspase-3 staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab205733, 1/2500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - HRP Anti-pro Caspase-3 antibody [E61] (ab205733)](https://www.abcam.com/ps/products/205/ab205733/Images/ab205733-265661-WBab2057331.jpg)
Western blot - HRP Anti-pro Caspase-3 antibody [E61] (ab205733)All lanes : HRP Anti-pro Caspase-3 antibody [E61] (ab205733) at 1/5000 dilution
Lane 1 :HeLa whole cell lysate (ab150035) at 10 µg
Lane 2 : Wild-type HAP1 cell lysate at 20 µg
Lane 3 : Wild-type HAP1 cell lysate Stuarosporine Treated (1µM for 4h) at 20 µg
Lane 4 : Caspase-3 knockout HAP1 cell lysate at 20 µg
Lane 5 : Caspase-3 knockout HAP1 cell lysate Stuarosporine Treated (1µM for 4h) at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205733 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-pro Caspase-3 antibody [E61] (ab205733)](https://www.abcam.com/ps/products/205/ab205733/Images/ab205733-4-benefits-of-recombinant-antibodies.png)
HRP Anti-pro Caspase-3 antibody [E61] (ab205733)
