Anti-Interferon regulatory factor 9/IRF-9 antibody (ab277803)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal to Interferon regulatory factor 9/IRF-9
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-Interferon regulatory factor 9/IRF-9 antibody
See all Interferon regulatory factor 9/IRF-9 primary antibodies -
Description
Rabbit recombinant multiclonal to Interferon regulatory factor 9/IRF-9 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Interferon regulatory factor 9/IRF-9 aa 186-201.
Database link: Q00978 -
Positive control
- ICC: Jurkat cells. WB: INF alpha treated Jurkat, MCF7 and PC-3 whole cell extracts.
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Interferon regulatory factor 9/IRF-9 antibody (ab277803)
Western blot analysis was performed on whole cell extracts (30 μg lysate) of Jurkat (human T cell leukemia cell line from peripheral blood) (Lane 1), Jurkat treated with IFN-alpha (10 ng/mL for 16hr) (Lane 2), MCF7 (human breast adenocarcinoma cell line) (Lane 3), MCF7 treated with IFN-alpha (10 ng/mL for 16hr) (Lane 4), PC3 (human prostate adenocarcinoma cell line) (Lane 5) and PC3 treated with IFN-alpha (10 ng/mL for 16hr) (Lane 6).
The blots were probed with ab277803 at 1-2 μg/mL and detected by chemiluminescence using a Goat anti-Rabbit IgG (H+L)-HRP secondary antibody, (0.4 μg/mL, 1/2500 dilution).
4-12% Bis-Tris gel.
The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed.
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Immunocytochemistry - Anti-Interferon regulatory factor 9/IRF-9 antibody (ab277803)Immunofluorescence was performed on fixed and permeabilized Jurkat cells for detection of endogenous IRF9 using ab277803 at 2 μ/mL and labeled with a Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate (1/2000).
Panel a) Cells that were stained for detection and localization of IRF9 protein (green).
Panel b) Nuclei (blue) using DAPI.
Panel c) Cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (1/300).
Panel d) Composite image of Panels a, b and c.
Panel e) Control cells with no primary antibody to assess background.
The images were captured at 60X magnification.
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Anti-Interferon regulatory factor 9/IRF-9 antibody (ab277803)