Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-11 antibody [EPR18628] (ab180673)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18628] to Caspase-11
  • Suitable for: WB, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Caspase-11 antibody [EPR18628]
    See all Caspase-11 primary antibodies

  • Description

    Rabbit monoclonal [EPR18628] to Caspase-11

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Untreated and treated with Lipopolysaccharide 10µg/ml for 8 hours RAW 264.7 whole cell lysate; YAC-1 and bEnd.3 whole cell lysates. IP: RAW 264.7 treated with Lipopolysaccharide 10µg/ml for 8 hours whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18628

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Caspase-11 antibody [EPR18628] (ab180673)
    Western blot - Anti-Caspase-11 antibody [EPR18628] (ab180673)
    All lanes : Anti-Caspase-11 antibody [EPR18628] (ab180673) at 1/1000 dilution

    Lane 1 : Untreated RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with Lipopolysaccharide 10µg/ml for 8 hours whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa
    Observed band size: 25,35,43 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Caspase-11 antibody [EPR18628] (ab180673)
    Western blot - Anti-Caspase-11 antibody [EPR18628] (ab180673)
    All lanes : Anti-Caspase-11 antibody [EPR18628] (ab180673) at 1/1000 dilution

    Lane 1 : YAC-1 (Mouse lymphoma cell line) whole cell lysate
    Lane 2 : bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa
    Observed band size: 25,35,43 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2: 3 minutes. 

  • Immunoprecipitation - Anti-Caspase-11 antibody [EPR18628] (ab180673)
    Immunoprecipitation - Anti-Caspase-11 antibody [EPR18628] (ab180673)

    Caspase-11 was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate with ab180673 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab180673 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: RAW 264.7 treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate, 10µg (Input).

    Lane 2: ab180673 IP in RAW 264.7 treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate.

    Lane 3: Rabbit  IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab180673 in RAW 264.7 treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-Caspase-11 antibody [EPR18628] (ab180673)
    Anti-Caspase-11 antibody [EPR18628] (ab180673)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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