Anti-Cleaved Caspase-9 antibody (ab2324)
Key features and details
- Rabbit polyclonal to Cleaved Caspase-9
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cleaved Caspase-9 antibody -
Description
Rabbit polyclonal to Cleaved Caspase-9 -
Host species
Rabbit -
Specificity
This antibody recognizes only the cleaved caspase-9 (37 kDa). It does not recognize full-length caspase-9 or any other caspase.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Positive control
- WB: Etoposide treated Jurkat whole cell lysate.
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General notes
Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase 9 (47 kDa) and Apaf-1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. This affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase 9 (47 kDa) and Apaf-1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. This affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cleaved Caspase-9 antibody (ab2324)All lanes : Anti-Cleaved Caspase-9 antibody (ab2324)
Lane 1 : Jurkat cell lysate, untreated
Lane 2 : Jurkat cell lysate, treated with etoposide for 6 hours
Observed band size: 37 kDa why is the actual band size different from the predicted?