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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-Prohibitin antibody [PHB/1882] (ab224657)

Anti-Prohibitin antibody [PHB/1882] (ab224657)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [PHB/1882] to Prohibitin
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Prohibitin antibody [PHB/1882]
    See all Prohibitin primary antibodies
  • Description

    Mouse monoclonal [PHB/1882] to Prohibitin
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Prohibitin aa 167-261. The exact sequence is proprietary.
    Database link: P35232

  • Positive control

    • IHC-P: Human liver tissue; WB: Ramos, HEK-293, HepG2, Jurkat, A549, K562, A431, and MCF7 cell lysates. Human liver tissue lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.05% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from bioreactor concentrate.
  • Clonality

    Monoclonal
  • Clone number

    PHB/1882
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [PHB/1882] (ab224657)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [PHB/1882] (ab224657)

    Formalin-fixed, paraffin-embedded human liver tissue stained for Prohibitin with ab224657 at 2 µg/ml in immunohistochemical analysis.

  • Western blot - Anti-Prohibitin antibody [PHB/1882] (ab224657)
    Western blot - Anti-Prohibitin antibody [PHB/1882] (ab224657)
    All lanes : Anti-Prohibitin antibody [PHB/1882] (ab224657) at 2 µg/ml

    Lane 1 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Predicted band size: 30 kDa

  • Western blot - Anti-Prohibitin antibody [PHB/1882] (ab224657)
    Western blot - Anti-Prohibitin antibody [PHB/1882] (ab224657)
    Anti-Prohibitin antibody [PHB/1882] (ab224657) at 2 µg/ml + Human liver tissue lysate

    Predicted band size: 30 kDa

  • Western blot - Anti-Prohibitin antibody [PHB/1882] (ab224657)
    Western blot - Anti-Prohibitin antibody [PHB/1882] (ab224657)
    All lanes : Anti-Prohibitin antibody [PHB/1882] (ab224657) at 1 µg/ml

    Lane 1 : A549 (human lung carcinoma cell line) cell lysate
    Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
    Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) cell lysate

    Developed using the ECL technique.

    Predicted band size: 30 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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