Anti-Prohibitin antibody [PHB/1882] (ab224657)
Key features and details
- Mouse monoclonal [PHB/1882] to Prohibitin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Prohibitin antibody [PHB/1882]
See all Prohibitin primary antibodies -
Description
Mouse monoclonal [PHB/1882] to Prohibitin -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Prohibitin aa 167-261. The exact sequence is proprietary.
Database link: P35232 -
Positive control
- IHC-P: Human liver tissue; WB: Ramos, HEK-293, HepG2, Jurkat, A549, K562, A431, and MCF7 cell lysates. Human liver tissue lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
PHB/1882 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Formalin-fixed, paraffin-embedded human liver tissue stained for Prohibitin with ab224657 at 2 µg/ml in immunohistochemical analysis.
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All lanes : Anti-Prohibitin antibody [PHB/1882] (ab224657) at 2 µg/ml
Lane 1 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Predicted band size: 30 kDa
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Anti-Prohibitin antibody [PHB/1882] (ab224657) at 2 µg/ml + Human liver tissue lysate
Predicted band size: 30 kDa
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All lanes : Anti-Prohibitin antibody [PHB/1882] (ab224657) at 1 µg/ml
Lane 1 : A549 (human lung carcinoma cell line) cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 30 kDa