Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP2804Y] to Prohibitin - Mitochondrial Marker
Suitable for: WB, IP, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker
See all Prohibitin primary antibodies
Description

Rabbit monoclonal [EP2804Y] to Prohibitin - Mitochondrial Marker
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Mouse Human
IHC-P	Mouse Rat Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Prohibitin aa 150-250. The exact sequence is proprietary.
Positive control
- WB: MCF-7, PC-12, A431 and Ramos cell lysate; IHC-P: human hepatocellular carcinoma tissue, mouse kidney tissue, mouse endometrium tissue, mouse liver tissue, rat liver tissue and rat stomach tissue; ICC/IF: Hela and Neuro-2a cells. IP: MCF7
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Monoclonal
Clone number

EP2804Y
Isotype

IgG
Research areas

Western blot - Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771)

All lanes : Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771) at 1/1000 dilution

Lane 1 : MCF-7 cell lysates
Lane 2 : PC-12 cell lysates
Lane 3 : A431 cell lysates
Lane 4 : Ramos cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 30 kDa
Observed band size: 30 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Prohibitin with ab75771 at 1/250.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Prohibitin with ab75711 at 1/250. An Alexa Fluor^® 488-conjugated anti-rabbit IgG was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling Prohibitin with ab75771 at 1/100. A HRP/AP polymerized secondary antibody was used.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771)

ab75771 (purified) at 1/110 dilution (20 µg/ml) immunoprecipitating Prohibitin in MCF7 whole cell lysate .
Lane 1 (input): MCF7(Human breast adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab75771 & MCF7 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab75771 in MCF7 whole cell lysate
For western blotting, ab75771 at 1/500 dilution (4.3 µg/ml) VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST .
Immunocytochemistry/ Immunofluorescence - Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771)

Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling Prohibitin with ab75711 at 1/250. An Alexa Fluor^® 488-conjugated anti-rabbit IgG was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse endometrium tissue labelling Prohibitin with ab75771 at 1/250.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling Prohibitin with ab75771 at 1/250.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling Prohibitin with ab75771 at 1/250.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue labelling Prohibitin with ab75771 at 1/250.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771)

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