Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2845Y] to RKIP (phospho S153)
- Suitable for: WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-RKIP (phospho S153) antibody [EP2845Y]
See all RKIP primary antibodies -
Description
Rabbit monoclonal [EP2845Y] to RKIP (phospho S153) -
Host species
Rabbit -
Specificity
ab75971 detects RKIP phosphorylated on serine 153. -
Tested Applications & Species
See all applications and species dataApplication Species WB RatHuman -
Immunogen
corresponding to Human RKIP (phospho S153).
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Positive control
- WB: C6 treated with 0.5uM TPA (12-O-tetradecanoylphorbol-13-acetate), HeLa treated with 100ng/ml PMA (Phorbol 12-myristate 13-acetate)
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2845Y -
Isotype
IgG -
Research areas
Images
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Lane 1 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution (Purified)
Lane 2 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100ng/ml PMA (Phorbol 12-myristate 13-acetate) for 48 hours whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking/Diluting buffer: 5% NFDM/TBST
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Lane 1 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution (Purified)
Lane 2 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 2 : C6 (Rat glial tumor glial cell) treated with 0.5uM TPA (12-O-tetradecanoylphorbol-13-acetate) for 3 hours whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking/Diluting buffer: 5% NFDM/TBST
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