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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971)

Price and availability

251 280 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2845Y] to RKIP (phospho S153)
  • Suitable for: WB
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-RKIP (phospho S153) antibody [EP2845Y]
    See all RKIP primary antibodies
  • Description

    Rabbit monoclonal [EP2845Y] to RKIP (phospho S153)
  • Host species

    Rabbit
  • Specificity

    ab75971 detects RKIP phosphorylated on serine 153.
  • Tested Applications & Species

    Application Species
    WB
    Rat
    Human
    See all applications and species data
  • Immunogen

    corresponding to Human RKIP (phospho S153).

  • Positive control

    • WB: C6 treated with 0.5uM TPA (12-O-tetradecanoylphorbol-13-acetate), HeLa treated with 100ng/ml PMA (Phorbol 12-myristate 13-acetate)
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP2845Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Neuroscience
    • Neurotransmitter
    • Acetylcholine
    • Neuroscience
    • Neurotransmitter
    • Neuropeptides
    • More Neuropeptides
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Other
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

Images

  • Western blot - Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971)
    Western blot - Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971)
    Lane 1 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution (Purified)
    Lane 2 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100ng/ml PMA (Phorbol 12-myristate 13-acetate) for 48 hours whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    Blocking/Diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971)
    Western blot - Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971)
    Lane 1 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution (Purified)
    Lane 2 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysates
    Lane 2 : C6 (Rat glial tumor glial cell) treated with 0.5uM TPA (12-O-tetradecanoylphorbol-13-acetate) for 3 hours whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    Blocking/Diluting buffer: 5% NFDM/TBST

  • Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971)
    Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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