Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TCEA1 antibody [EPR15036] (ab184158)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15036] to TCEA1
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-TCEA1 antibody [EPR15036]
    See all TCEA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15036] to TCEA1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, HEK293, K562 cell lysates; HUVEC cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TCEA1 antibody [EPR15036] (ab184158)
    Western blot - Anti-TCEA1 antibody [EPR15036] (ab184158)
    All lanes : Anti-TCEA1 antibody [EPR15036] (ab184158) at 1/50000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR15036] (ab184158)
    Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR15036] (ab184158)

    Immunofluorescence analysis of paraformaldehyde-fixed HUVEC cells, staining TCEA1 (red) with ab184158 at 1/250 dilution. Alexa Fluor®555-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunoprecipitation - Anti-TCEA1 antibody [EPR15036] (ab184158)
    Immunoprecipitation - Anti-TCEA1 antibody [EPR15036] (ab184158)

    Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate, labeling TCEA1 immunoprecipitated using ab184158 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-TCEA1 antibody [EPR15036] (ab184158)
    Anti-TCEA1 antibody [EPR15036] (ab184158)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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