Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14820(B)] to TCEA1
  • Suitable for: WB, ICC/IF, IP, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-TCEA1 antibody [EPR14820(B)]
    See all TCEA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14820(B)] to TCEA1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • K562, HeLa, and SP2/0 cell lysate. Human lung adenocarcinoma and colon tissue. HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
    Western blot - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
    All lanes : Anti-TCEA1 antibody [EPR14820(B)] (ab184181) at 1/10000 dilution

    Lane 1 : K562 lysate
    Lane 2 : HeLa lysate
    Lane 3 : SP2/0 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa

  • Immunoprecipitation - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
    Immunoprecipitation - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

    Immunoprecipitation analysis of HeLa cell lysate labeling TCEA1 with ab184181 at 1/50 dilution.  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated used as secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

    Immunohistochemistry analysis of Human lung adenocarcinoma tissue labeling TCEA1 with ab184181 at 1/100 dilution. Secondary antibody HRP conjugated Rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

    Immunohistochemistry analysis of Human colon tissue labeling TCEA1 with ab184181 at 1/100 dilution. Secondary antibody HRP conjugated Rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
    Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

    Immunocytochemistry analysis of a HeLa cells labeling TCEA1 with ab184181 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) at a 1/200 dilution. Dapi counterstain shown in blue.

  • Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
    Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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