Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14820(B)] to TCEA1
Suitable for: WB, ICC/IF, IP, IHC-P
Reacts with: Mouse, Human

Product name

Anti-TCEA1 antibody [EPR14820(B)]
See all TCEA1 primary antibodies
Description

Rabbit monoclonal [EPR14820(B)] to TCEA1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- K562, HeLa, and SP2/0 cell lysate. Human lung adenocarcinoma and colon tissue. HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR14820(B)
Isotype

IgG
Research areas

Western blot - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

All lanes : Anti-TCEA1 antibody [EPR14820(B)] (ab184181) at 1/10000 dilution

Lane 1 : K562 lysate
Lane 2 : HeLa lysate
Lane 3 : SP2/0 lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa
Immunoprecipitation - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

Immunoprecipitation analysis of HeLa cell lysate labeling TCEA1 with ab184181 at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated used as secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

Immunohistochemistry analysis of Human lung adenocarcinoma tissue labeling TCEA1 with ab184181 at 1/100 dilution. Secondary antibody HRP conjugated Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

Immunohistochemistry analysis of Human colon tissue labeling TCEA1 with ab184181 at 1/100 dilution. Secondary antibody HRP conjugated Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

Immunocytochemistry analysis of a HeLa cells labeling TCEA1 with ab184181 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) at a 1/200 dilution. Dapi counterstain shown in blue.
Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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