Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-TCEA1 antibody [EPR14821] (ab185947)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14821] to TCEA1
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
Reacts with: Mouse, Human

Product name

Anti-TCEA1 antibody [EPR14821]
See all TCEA1 primary antibodies
Description

Rabbit monoclonal [EPR14821] to TCEA1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- K562, HeLa, SP2/0 and HUVEC cell lysate; Human bladder transitional cell carcinoma tissue; HeLa, HUVEC cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14821
Isotype

IgG
Research areas

Western blot - Anti-TCEA1 antibody [EPR14821] (ab185947)

All lanes : Anti-TCEA1 antibody [EPR14821] (ab185947) at 1/10000 dilution

Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SP2/0 cell lysate
Lane 4 : HUVEC cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14821] (ab185947)

Immunohistochemical analysis of paraffin embedded Human bladder transitional cell carcinoma tissue labeling TCEA1 with ab185947 at 1/500 dilution. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR14821] (ab185947)

Immunofluorescent staining of -20 °C acetone fixed HeLa cells labeling TCEA1 with ab185947 at 1/500 dilution (red; left image) and DAPI nuclear staining (blue; right image). Secondary staining with Goat anti Rabbit IgG (Alexa Fluor^® 555) at a 1/200 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR14821] (ab185947)

Immunofluorescent staining of 4% paraformaldehyde fixed HUVEC cells labeling TCEA1 with ab185947 at 1/500 dilution (red; left image) and DAPI nuclear staining (blue; right image). Secondary staining with Goat anti Rabbit IgG (Alexa Fluor^® 555) at a 1/200 dilution.
Flow Cytometry - Anti-TCEA1 antibody [EPR14821] (ab185947)

Flow cytometric analysis of 2% paraformaldehyde HeLa cells labeling TCEA1 with ab185947 at 1/250 dilution (red) compared to a Rabbit monoclonal IgG isotype control antibody (green). Secondary staining with Goat anti Rabbit IgG (FITC) at a 1/150 dilution.
Immunoprecipitation - Anti-TCEA1 antibody [EPR14821] (ab185947)

Immunoprecipitation analysis of HeLa cell lysate labeling TCEA1 with ab185947 at 1/50 dilution. Secondary staining with Goat anti Rabbit IgG (H+L) Peroxidase conjugated antibody at a 1/1000 dilution.
Anti-TCEA1 antibody [EPR14821] (ab185947)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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