Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TCEA1 antibody [EPR14821] (ab185947)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14821] to TCEA1
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-TCEA1 antibody [EPR14821]
    See all TCEA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14821] to TCEA1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • K562, HeLa, SP2/0 and HUVEC cell lysate; Human bladder transitional cell carcinoma tissue; HeLa, HUVEC cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TCEA1 antibody [EPR14821] (ab185947)
    Western blot - Anti-TCEA1 antibody [EPR14821] (ab185947)
    All lanes : Anti-TCEA1 antibody [EPR14821] (ab185947) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : SP2/0 cell lysate
    Lane 4 : HUVEC cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14821] (ab185947)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14821] (ab185947)

    Immunohistochemical analysis of paraffin embedded Human bladder transitional cell carcinoma tissue labeling TCEA1 with ab185947 at 1/500 dilution. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR14821] (ab185947)
    Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR14821] (ab185947)

    Immunofluorescent staining of -20 °C acetone fixed HeLa cells labeling TCEA1 with ab185947 at 1/500 dilution (red; left image) and  DAPI nuclear staining (blue; right image).  Secondary staining with Goat anti Rabbit IgG (Alexa Fluor® 555) at a 1/200 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR14821] (ab185947)
    Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR14821] (ab185947)

    Immunofluorescent staining of 4% paraformaldehyde fixed HUVEC cells labeling TCEA1 with ab185947 at 1/500 dilution (red; left image) and  DAPI nuclear staining (blue; right image).  Secondary staining with Goat anti Rabbit IgG (Alexa Fluor® 555) at a 1/200 dilution.

  • Flow Cytometry - Anti-TCEA1 antibody [EPR14821] (ab185947)
    Flow Cytometry - Anti-TCEA1 antibody [EPR14821] (ab185947)

    Flow cytometric analysis of 2% paraformaldehyde HeLa cells labeling TCEA1 with ab185947 at 1/250 dilution (red) compared to a Rabbit monoclonal IgG isotype control antibody (green).  Secondary staining with Goat anti Rabbit IgG (FITC) at a 1/150 dilution.

  • Immunoprecipitation - Anti-TCEA1 antibody [EPR14821] (ab185947)
    Immunoprecipitation - Anti-TCEA1 antibody [EPR14821] (ab185947)

    Immunoprecipitation analysis of HeLa cell lysate labeling TCEA1 with ab185947 at 1/50 dilution.  Secondary staining with Goat anti Rabbit IgG (H+L) Peroxidase conjugated antibody at a 1/1000 dilution.

  • Anti-TCEA1 antibody [EPR14821] (ab185947)
    Anti-TCEA1 antibody [EPR14821] (ab185947)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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