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Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6828(B)] to Mannose Receptor
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Mannose Receptor antibody [EPR6828(B)]
    See all Mannose Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR6828(B)] to Mannose Receptor

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Mannose Receptor aa 1400 to the C-terminus. The exact sequence is proprietary.
    Database link: P22897

  • Positive control

    • WB: Human lung tissue; ICC/IF: MOLT-4 (human lymphoblastic leukemia cell line); Flow Cyt: MOLT-4 (human lymphoblastic leukemia cell line).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 1.75 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR6828(B)

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028)
    Immunocytochemistry/ Immunofluorescence - Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028)

    Immunocytochemistry/Immunofluorescence analysis MOLT-4 (Human lymphoblastic leukemia) cells labeling Mannose Receptor with ab125028 at 1 μg/mL. Cells were fixed with 100% methanol. ab150081, an AlexaFluor®488 Goat anti-Rabbit at 1/1000 was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/250) - Microtubule Marker (Alexa Fluor® 594). Nuclei were counterstained with DAPI (blue).

    Confocal image showing membranous staining on MOLT-4 cell line.

  • Flow Cytometry - Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028)
    Flow Cytometry - Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028)

    Flow Cytometry analysis of Molt-4 (human acute lymphoblastic leukemia ) cells labeling Mannose Receptor with unpurified ab125028 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028)
    Western blot - Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028)
    All lanes : Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028) at 1/1000 dilution

    Lane 1 : Human lung tissue lysate
    Lane 2 : Human brain tissue lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
    Lane 5 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 166 kDa
    Observed band size: 200 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 160 kDa (possible non-specific binding), 65 kDa (possible non-specific binding)


    Exposure time: 20 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed in U-937 and THP-1 is consistent with the literature (PMID: 20801507 and PMID: 30591689). Rabbit monoclonal [EPR16891] to GAPDH (ab181602) used as loading control.

  • OI-RD Scanning - Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028)
    OI-RD Scanning - Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028)
    Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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