Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22489-7] to Mannose Receptor
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Mannose Receptor antibody [EPR22489-7]
    See all Mannose Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR22489-7] to Mannose Receptor

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1 treated with 20 ng/ml PMA for 24 hours, then treated with 20ng/ml IL-4 for 48 hours, whole cell lysate; human liver, lymph node, lung and placenta lysates. IHC-P: Human liver, colon, and Karposi's sarcoma tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22489-7

  • Isotype

    IgG1

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Mannose Receptor with ab252921 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in macrophages (Kupffer cells) of human liver (PMID: 26938527) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)

    Immunohistochemical analysis of paraffin-embedded human Karposi's sarcoma tissue labeling Mannose Receptor with ab252921 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in tumor cells of human Kaposi’s Sarcoma (PMID: 9060831) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Mannose Receptor with ab252921 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in macrophages of human colon (PMID: 9060831) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)
    Western blot - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)
    All lanes : Anti-Mannose Receptor antibody [EPR22489-7] (ab252921) at 1/1000 dilution

    Lane 1 : Human liver lysate
    Lane 2 : Human lung lysate
    Lane 3 : Human lymph node lysate
    Lane 4 : Human placenta lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 166 kDa
    Observed band size: 175 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 mins. Lanes 2 & 3: 26 secs. Lane 4: 10 secs.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 26202986; 23752129).

  • Western blot - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)
    Western blot - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)
    All lanes : Anti-Mannose Receptor antibody [EPR22489-7] (ab252921) at 1/1000 dilution

    Lane 1 : Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate
    Lane 2 : THP-1 treated with 20 ng/ml PMA for 24 hours, then treated with 20ng/ml IL-4 for 48 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 166 kDa
    Observed band size: 175 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight of 175 kDa in lane 2 observed is consistent with what has been described in the literature (PMID: 26202986; 23752129). This antibody also reacts with an unidentifiable protein around 40 kDa.

     

  • Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)
    Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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