Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22489-7] to Mannose Receptor
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-Mannose Receptor antibody [EPR22489-7]
See all Mannose Receptor primary antibodies
Description

Rabbit monoclonal [EPR22489-7] to Mannose Receptor
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: THP-1 treated with 20 ng/ml PMA for 24 hours, then treated with 20ng/ml IL-4 for 48 hours, whole cell lysate; human liver, lymph node, lung and placenta lysates. IHC-P: Human liver, colon, and Karposi's sarcoma tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22489-7
Isotype

IgG1
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Mannose Receptor with ab252921 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in macrophages (Kupffer cells) of human liver (PMID: 26938527) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)

Immunohistochemical analysis of paraffin-embedded human Karposi's sarcoma tissue labeling Mannose Receptor with ab252921 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in tumor cells of human Kaposi’s Sarcoma (PMID: 9060831) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Mannose Receptor with ab252921 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in macrophages of human colon (PMID: 9060831) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)

All lanes : Anti-Mannose Receptor antibody [EPR22489-7] (ab252921) at 1/1000 dilution

Lane 1 : Human liver lysate
Lane 2 : Human lung lysate
Lane 3 : Human lymph node lysate
Lane 4 : Human placenta lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 166 kDa
Observed band size: 175 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 3 mins. Lanes 2 & 3: 26 secs. Lane 4: 10 secs.

The molecular weight observed is consistent with what has been described in the literature (PMID: 26202986; 23752129).
Western blot - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)

All lanes : Anti-Mannose Receptor antibody [EPR22489-7] (ab252921) at 1/1000 dilution

Lane 1 : Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 2 : THP-1 treated with 20 ng/ml PMA for 24 hours, then treated with 20ng/ml IL-4 for 48 hours, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 166 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight of 175 kDa in lane 2 observed is consistent with what has been described in the literature (PMID: 26202986; 23752129). This antibody also reacts with an unidentifiable protein around 40 kDa.
Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)

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