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Neuroscience Neurology process Neural Signal Transduction

Anti-14-3-3 antibody [EPR5872] (ab133538)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-14-3-3 antibody [EPR5872] (ab133538)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5872] to 14-3-3
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-14-3-3 antibody [EPR5872]
    See all 14-3-3 primary antibodies
  • Description

    Rabbit monoclonal [EPR5872] to 14-3-3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human 14-3-3 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • Fetal lung, A549, Human kidney, and Human spleen lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.18 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5872
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • 14-3-3

Images

  • Western blot - Anti-14-3-3 antibody [EPR5872] (ab133538)
    Western blot - Anti-14-3-3 antibody [EPR5872] (ab133538)
    All lanes : Anti-14-3-3 antibody [EPR5872] (ab133538) at 1/1000 dilution

    Lane 1 : Human fetal lung lysate
    Lane 2 : A549 cell lysate
    Lane 3 : Human kidney lysate
    Lane 4 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 27 kDa

  • OI-RD Scanning - Anti-14-3-3 antibody [EPR5872] (ab133538)
    OI-RD Scanning - Anti-14-3-3 antibody [EPR5872] (ab133538)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-14-3-3 antibody [EPR5872] (ab133538)
    Anti-14-3-3 antibody [EPR5872] (ab133538)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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