Anti-14-3-3 (phospho S58) antibody (ab30554)
Key features and details
- Rabbit polyclonal to 14-3-3 (phospho S58)
- Suitable for: WB, IHC-Fr
- Reacts with: Mouse, Rat, Zebrafish
- Isotype: IgG
Overview
-
Product name
Anti-14-3-3 (phospho S58) antibody
See all 14-3-3 primary antibodies -
Description
Rabbit polyclonal to 14-3-3 (phospho S58) -
Host species
Rabbit -
Specificity
Specific for the ~29k 14-3-3 protein phosphorylated at Ser58. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
The N-terminal sequence used as antigen has high homology across a wide variety of species for all of the 14-3-3 proteins. We have not specifically tested it for reactivity against each protein in any given species, we do expect it to recognize most all of them.
-
Tested applications
Suitable for: WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Zebrafish
Predicted to work with: Sheep, Chicken, Cow, Dog, Xenopus laevis, Non human primates
-
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phosphorylation site Ser58 of rat 14-3-3 protein.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-14-3-3 (phospho S58) antibody (ab30554)All lanes : Anti-14-3-3 (phospho S58) antibody (ab30554) at 1/1000 dilution
Lane 1 : Rat brain stem lysate
Lane 2 : Rat brain stem lysate with the phosphopeptide used as the antigen
Observed band size: 29 kDa why is the actual band size different from the predicted?
-
Immunohistochemistry (Frozen sections) - Anti-14-3-3 (phospho S58) antibody (ab30554)ab30554 staining 14-3-3 (phospho S58) in Mouse hippocampal tissue by Immunohistochemistry (Frozen sections). ab30554 is green, red staining is GFAP and nuclei are stained blue with DAPI. -
Western blot - Anti-14-3-3 (phospho S58) antibody (ab30554)All lanes : Anti-14-3-3 (phospho S58) antibody (ab30554) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate (20ug)
Lane 3 : Mouse brain homogenate (20ug)
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
