Anti-Hsp60 antibody [LK1] (ab233878)
![Anti-Hsp60 antibody [LK1] (ab233878)](https://www.abcam.com/ps/products/233/ab233878/Images/ab233878-307832-HSP60-IHC1min.jpg "Anti-Hsp60 antibody [LK1] (ab233878)")
Key features and details
- Mouse monoclonal [LK1] to Hsp60
- Suitable for: WB, Flow Cyt, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Hsp60 antibody [LK1]
See all Hsp60 primary antibodies -
Description
Mouse monoclonal [LK1] to Hsp60 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC HumanIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human Hsp60.
Database link: P10809 -
Positive control
- IHC-P: Human breast carcinoma and pancreas tissues; WB: HeLa cell lysate; ICC: MCF7 cells; Flow Cyt: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
LK1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (ab233878)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Hsp60 using ab233878 at 1 μg/ml in immunohistochemical analysis.
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![Western blot - Anti-Hsp60 antibody [LK1] (ab233878)](https://www.abcam.com/ps/products/233/ab233878/Images/ab233878-456414-anti-hsp60-antibody-lk1-western-blot-hela-human.jpg)
Western blot - Anti-Hsp60 antibody [LK1] (ab233878)Anti-Hsp60 antibody [LK1] (ab233878) at 0.5 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 61 kDa
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![Immunocytochemistry - Anti-Hsp60 antibody [LK1] (ab233878)](https://www.abcam.com/ps/products/233/ab233878/Images/ab233878-456412-anti-hsp60-antibody-lk1-immunocytochemistry-mcf7-human.jpg)
Immunocytochemistry - Anti-Hsp60 antibody [LK1] (ab233878)Immunocytochemistry analysis of PFA-fixed MCF-7 (human breast adenocarcinoma cell line) cells labeling Hsp60 with ab233878 at 2 µg/mL followed by goat anti-Mouse IgG-CF488 (green). Nuclei are labeled with Reddot (red).
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![Flow Cytometry - Anti-Hsp60 antibody [LK1] (ab233878)](https://www.abcam.com/ps/products/233/ab233878/Images/ab233878-456413-anti-hsp60-antibody-lk1-flow-cytometry-hela-human.jpg)
Flow Cytometry - Anti-Hsp60 antibody [LK1] (ab233878)Flow Cytometry analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Hsp60 with ab233878 at 2 µg/106 cells followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (ab233878)](https://www.abcam.com/ps/products/233/ab233878/Images/ab233878-307833-HSP60-IHC2min.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (ab233878)Formalin-fixed, paraffin-embedded human pancreas tissue stained for Hsp60 using ab233878 at 1 μg/ml in immunohistochemical analysis.
