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Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)

Key features and details

  • Mouse monoclonal [USC127-3] to Hsp60 - Mitochondrial Marker
  • Suitable for: IHC-P, Flow Cyt, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker
    See all Hsp60 primary antibodies

  • Description

    Mouse monoclonal [USC127-3] to Hsp60 - Mitochondrial Marker

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • HepG2, Hela, Jurkat, HDFn, Hek293T cell lysates; Human Heart Homogenate lysate; Human Liver mitochondrial lysate; HDFn cells; Hela cells

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)
    Western blot - Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)
    All lanes : Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567) at 1 µg/ml

    Lane 1 : Ladder
    Lane 2 : HepG2 cell lysate at 10 µg
    Lane 3 : Hela cell lysate at 10 µg
    Lane 4 : Jurkat cell lysate at 10 µg
    Lane 5 : HDFn cell lysate at 10 µg
    Lane 6 : Hek293T cell lysate at 10 µg
    Lane 7 : Human Heart Homogenate lysate at 10 µg
    Lane 8 : Human Liver mitochondrial lysate at 10 µg

    Predicted band size: 61 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)
    Immunofluorescent staining of Hsp60 in HDFn cells (human) using ab128567.
    The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were then incubated with the antibody (ab128567, 1µg/ml) for 2h at room temperature or overnight at 4°C. The secondary antibody was (Green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix.
  • Flow Cytometry - Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)
    Flow Cytometry - Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)
    ab128567 staining Hsp60 in Hela cells by Flow cytometry.
    Hela cells were fixed and permeabilized with methanol and stained with 5µg/mL Hsp60 antibody (ab128567) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry. 1% BSA was used as the blocking reagent for all the blocking steps.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)
    IHC image of Hsp60 staining in human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab128567, 0.2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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