Human Apolipoprotein CI ELISA Kit (APOCI) (ab108808)
Key features and details
- Sensitivity: 0.04 µg/ml
- Range: 0.063 µg/ml - 4 µg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Apolipoprotein CI ELISA Kit (APOCI) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4% Inter-assay Sample n Mean SD CV% Overall 9.6% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.04 µg/ml -
Range
0.063 µg/ml - 4 µg/ml -
Recovery
98 %
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Assay time
5h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Apolipoprotein CI (APOCI) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein CI concentrations in plasma, serum, cell lysates and cell culture supernatants.
An Apolipoprotein CI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein CI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein CI captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Apolipoprotein CI Antibody 1 x 120µl Apolipoprotein CI Microplate (12 x 8 well strips) 1 unit Apolipoprotein CI Standard 2 vials Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
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Function
Appears to modulate the interaction of APOE with beta-migrating VLDL and inhibit binding of beta-VLDL to the LDL receptor-related protein. Binds free fatty acids and reduces their intracellular esterification. -
Tissue specificity
Synthesized mainly in liver and to a minor degree in intestine. Secreted in plasma. -
Sequence similarities
Belongs to the apolipoprotein C1 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- APO C1
- Apo CI
- Apo-CIB
see all -
Database links
- Entrez Gene: 341 Human
- Omim: 107710 Human
- SwissProt: P02654 Human
- Unigene: 110675 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab108808