Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SPAK antibody [EPR6394] (ab128894)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6394] to SPAK
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SPAK antibody [EPR6394]
    See all SPAK primary antibodies

  • Description

    Rabbit monoclonal [EPR6394] to SPAK

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human SPAK aa 400-500.
    Database link: Q9UEW8

  • Positive control

    • SH-SY5Y, 293T, Jurkat, BxPC3 and HepG2 whole cell lysate (ab7900); Human colon tissue

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as STK39

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SPAK antibody [EPR6394] (ab128894)
    Western blot - Anti-SPAK antibody [EPR6394] (ab128894)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: SPAK knockout HAP1 whole cell lysate (20 µg)
    Lane 3: Jurkat whole cell lysate (20 µg)
    Lane 4: MCF7 whole cell lysate (20 µg)


    Lanes 1 - 4: Merged signal (red and green). Green - ab128894 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab128894 was shown to specifically react with SPAK in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when SPAK knockout samples were uexamined. Wild-type and SPAK knockout samples were subjected to SDS-PAGE. Ab128894 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-SPAK antibody [EPR6394] (ab128894)
    Western blot - Anti-SPAK antibody [EPR6394] (ab128894)
    All lanes : Anti-SPAK antibody [EPR6394] (ab128894) at 1/1000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : BxPC3 cell lysate
    Lane 5 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 59 kDa
    Observed band size: 65 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAK antibody [EPR6394] (ab128894)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAK antibody [EPR6394] (ab128894)

    ab128894, at 1/50 dilution, staining SPAK in paraffin embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-SPAK antibody [EPR6394] (ab128894)
    OI-RD Scanning - Anti-SPAK antibody [EPR6394] (ab128894)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-SPAK antibody [EPR6394] (ab128894)
    Anti-SPAK antibody [EPR6394] (ab128894)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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