Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19824] to Casein Kinase 1 alpha
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Casein Kinase 1 alpha antibody [EPR19824]
    See all Casein Kinase 1 alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR19824] to Casein Kinase 1 alpha

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T, HeLa, MCF7 and NIH/3T3 whole cell lysates; Human fetal brain, fetal kidney, fetal spleen and skin lysates; Mouse and rat brain, kidney, spleen and skin lysates. IP: Human skin lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)
    Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)
    All lanes : Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HEK-293T Casein Kinase 1 alpha KO whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 39 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The lysates were kindly provided by our collaborator Dr. Yi Rao, Peking University.

  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)
    Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)
    All lanes : Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human fetal spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Predicted band size: 39 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)
    Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)
    All lanes : Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 39 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)
    Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)
    All lanes : Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat kidney lysate
    Lane 3 : Rat spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Rabbit monoclonal [EPR19824] to Casein Kinase 1 alpha (ab206652) at 1/10000 dilution

    Predicted band size: 39 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)
    Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)
    All lanes : Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/1000 dilution

    Lane 1 : Human skin lysate
    Lane 2 : Mouse skin lysate
    Lane 3 : Rat skin lysate
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 39 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/2: 15 seconds; Lane 3: 3 min; Lane 4/5/6: 1 second.

  • Immunoprecipitation - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)
    Immunoprecipitation - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)

    Casein Kinase 1 alpha was immunoprecipitated from 0.35 mg of Human skin lysate with ab206652 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab206652 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human skin lysate, 10µg (Input).

    Lane 2: ab206652 IP in Human skin lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206652 in Human skin lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)
    Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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