Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1961(2)] to Casein Kinase 1 alpha
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Casein Kinase 1 alpha antibody [EPR1961(2)]
    See all Casein Kinase 1 alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR1961(2)] to Casein Kinase 1 alpha

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF-7, HeLa, Jurkat, Rat brain lysates, RAW 264.7, PC-12 and HT-1376 cell lysates

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)
    Western blot - Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)
    All lanes : Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296) at 1/1000 dilution (purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
    Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)
    Western blot - Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)
    Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296) at 1/2000 dilution + RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)
    Western blot - Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)
    All lanes : Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296) at 1/1000 dilution (purified)

    Lane 1 : Rat brain lysates
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)
    Western blot - Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)
    All lanes : Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296) at 1/1000 dilution (unpurified)

    Lane 1 : MCF7 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HT-1376 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 39 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?

  • Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)
    Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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