Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)
![Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)](https://www.abcam.com/ps/products/108/ab108296/Images/ab108296-293451-anti-casein-kinase-1-alpha-antibody-epr19612-western-blot.jpg "Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1961(2)] to Casein Kinase 1 alpha
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Casein Kinase 1 alpha antibody [EPR1961(2)]
See all Casein Kinase 1 alpha primary antibodies -
Description
Rabbit monoclonal [EPR1961(2)] to Casein Kinase 1 alpha -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF-7, HeLa, Jurkat, Rat brain lysates, RAW 264.7, PC-12 and HT-1376 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1961(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)All lanes : Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296) at 1/1000 dilution (purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)](https://www.abcam.com/ps/products/108/ab108296/Images/ab108296-293452-anti-casein-kinase-1-alpha-antibody-epr19612-western-blot.jpg)
Western blot - Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296) at 1/2000 dilution + RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)](https://www.abcam.com/ps/products/108/ab108296/Images/ab108296-293450-anti-casein-kinase-1-alpha-antibody-epr19612-western-blot.jpg)
Western blot - Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)All lanes : Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296) at 1/1000 dilution (purified)
Lane 1 : Rat brain lysates
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDaBlocking and diluting buffer: 5% NFDM/TBST
-
![Western blot - Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)](https://www.abcam.com/ps/products/108/ab108296/Images/ab108296-120804-anti-casein-kinase-1-alpha-antibody-epr19612-western-blot.jpg)
Western blot - Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)All lanes : Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296) at 1/1000 dilution (unpurified)
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HT-1376 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
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![Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)](https://www.abcam.com/ps/products/108/ab108296/Images/ab108296-5-benefits-of-recombinant-antibodies.png)
Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)
