Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Wednesday March 03, 2021

Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10435] to Protective protein/Cathepsin A (PPCA)
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435]
See all Protective protein/Cathepsin A (PPCA) primary antibodies
Description

Rabbit monoclonal [EPR10435] to Protective protein/Cathepsin A (PPCA)
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa cells; HepG2, 293, Jurkat and HeLa whole cell lysate (ab150035).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10435
Isotype

IgG
Research areas

Western blot - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553)

All lanes : Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553) at 1/20000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : 293 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 54 kDa
Observed band size: 54 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Protective protein/Cathepsin A (PPCA) with ab184553 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Flow Cytometry - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553)

Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Protective protein/Cathepsin A (PPCA) with ab184553 at 1/140 dilution (red) compared with a Rabbit monoclonal IgG isotype control (green), followed by Goat anti-rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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