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Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10435] to Protective protein/Cathepsin A (PPCA)
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435]
    See all Protective protein/Cathepsin A (PPCA) primary antibodies

  • Description

    Rabbit monoclonal [EPR10435] to Protective protein/Cathepsin A (PPCA)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa cells; HepG2, 293, Jurkat and HeLa whole cell lysate (ab150035).

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553)
    Western blot - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553)
    All lanes : Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553) at 1/20000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553)
    Immunocytochemistry/ Immunofluorescence - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Protective protein/Cathepsin A (PPCA) with ab184553 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Flow Cytometry - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553)
    Flow Cytometry - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Protective protein/Cathepsin A (PPCA) with ab184553 at 1/140 dilution (red) compared with a Rabbit monoclonal IgG isotype control (green), followed by Goat anti-rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553)
    Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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