Anti-Casein Kinase 1 alpha antibody [EPR19824] - BSA and Azide free (ab223144)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19824] to Casein Kinase 1 alpha - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Casein Kinase 1 alpha antibody [EPR19824] - BSA and Azide free
See all Casein Kinase 1 alpha primary antibodies -
Description
Rabbit monoclonal [EPR19824] to Casein Kinase 1 alpha - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T, HeLa, MCF7 and NIH/3T3 whole cell lysates; Human fetal brain, fetal kidney, fetal spleen and skin lysates; Mouse and rat brain, kidney, spleen and skin lysates. IP: Human skin lysate.
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General notes
ab223144 is the carrier-free version of ab206652.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19824 -
Isotype
IgG -
Research areas
Images
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Casein Kinase 1 alpha was immunoprecipitated from 0.35 mg of Human skin lysate with ab206652 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab206652 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Human skin lysate, 10µg (Input).
Lane 2: ab206652 IP in Human skin lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206652 in Human skin lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206652).
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All lanes : Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HEK-293T Casein Kinase 1 alpha KO whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206652).
Blocking/Dilution buffer: 5% NFDM/TBST.
The lysates were kindly provided by our collaborator Dr. Yi Rao, Peking University.
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