Anti-cGMP antibody [EPR10355] (ab169753)
![Anti-cGMP antibody [EPR10355] (ab169753)](https://www.abcam.com/ps/products/169/ab169753/Images/ab169753-168419-anti-cgmp-antibody-epr10355-elisa.jpg "Anti-cGMP antibody [EPR10355] (ab169753)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10355] to cGMP
- Suitable for: ELISA
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-cGMP antibody [EPR10355]
See all cGMP primary antibodies -
Description
Rabbit monoclonal [EPR10355] to cGMP -
Host species
Rabbit -
Tested applications
Suitable for: ELISAmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P,IP or WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Chemical/ Small Molecule corresponding to cGMP conjugated to keyhole limpet haemocyanin.
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General notes
The product is specific for cGMP and as tested it does not cross-react with cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP and cCMP.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10355 -
Isotype
IgG -
Research areas
Images
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ELISA - Anti-cGMP antibody [EPR10355] (ab169753)2 µg/ml of Goat anti-rabbit IgG (50 µl/well) was coated into 96-wells. After washing with 0.1 % Tween-20, 0.1 µg/ml of ab169753 (50 µl/well) was added and incubated for 1 hour. After washing, 0, 8, 48, 64, 96, 128, 256 and 1000 ng/ml of cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP and cCMP (25 µl/well) were added along with 25 µl of the 1/50 diluted HRP conjugated cGMP. After washing, TMB was used to develop the color.
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![ELISA - Anti-cGMP antibody [EPR10355] (ab169753)](https://www.abcam.com/ps/products/169/ab169753/Images/ab169753-168418-anti-cgmp-antibody-epr10355-elisa.png)
ELISA - Anti-cGMP antibody [EPR10355] (ab169753)2µg/ml of goat-anti-rabbit IgG (50µl/well) was coated into 96-wells. After washing with 0.1% Tween-20, 0.2µg/ml of ab169753 (50 µl / well) was added and incubated for 1hr. Then 62.5ng/ml of cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP, cCMP and H2O (25 µl / well each) were added, respectively. Finally 25 µl of 1/50 diluted HRP conjugated cGMP was added in each well. TMB was used to develop the color. -
![Anti-cGMP antibody [EPR10355] (ab169753)](https://www.abcam.com/ps/products/169/ab169753/Images/ab169753-3-benefits-of-recombinant-antibodies.png)
Anti-cGMP antibody [EPR10355] (ab169753)