Anti-Casein Kinase 1 alpha (phospho Y294) antibody (ab192650)
Key features and details
- Rabbit polyclonal to Casein Kinase 1 alpha (phospho Y294)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Casein Kinase 1 alpha (phospho Y294) antibody
See all Casein Kinase 1 alpha primary antibodies -
Description
Rabbit polyclonal to Casein Kinase 1 alpha (phospho Y294) -
Host species
Rabbit -
Specificity
ab192650 detects endogenous levels of Casein Kinase 1 alpha only when phosphorylated at Tyrosine 294. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Casein Kinase 1 alpha (phospho Y264) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. [Peptide sequence around phosphorylation site of tyrosine 294 (Y-D-Y(p)-T-F)].
Database link: P48729 -
Positive control
- HT29 cell extract treated with heat shock; Human brain tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab192650 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibody was removed by chromatogramphy using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Casein Kinase 1 alpha (phospho Y294) antibody (ab192650)All lanes : Anti-Casein Kinase 1 alpha (phospho Y294) antibody (ab192650) at 1/500 dilution
Lane 1 : HT29 cell extract treated with heat shock
Lane 2 : HT29 cell extract treated with heat shock with antigen-specific peptide
Predicted band size: 39 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 1 alpha (phospho Y294) antibody (ab192650)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Casein Kinase 1 alpha (phospho Y294) with ab192650 at 1/50 dilution (left image), or the same antibody preincubated with blocking peptide (right image).
