Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (ab251356)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19561] to Histone H4 (mono methyl R78) - BSA and Azide free
  • Suitable for: Flow Cyt, Dot blot, ICC, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free
    See all Histone H4 primary antibodies

  • Description

    Rabbit monoclonal [EPR19561] to Histone H4 (mono methyl R78) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, Dot blot, ICC, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251356 is the carrier-free version of ab202036. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251356 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR19561

  • Isotype

    IgG

Images

  • Western blot - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (ab251356)
    Western blot - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (ab251356)
    All lanes : Anti-Histone H4 (mono methyl R78) antibody [EPR19561] (ab202036) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma ) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 3 minutes


    This data was developed using ab202036, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (ab251356)
    Dot Blot - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (ab251356)
    This data was developed using ab202036, the same antibody clone in a different buffer formulation.Dot blot analysis of Histone H4 (mono methyl R78) labeled with ab202036 at 1/1000 dilution. Lane 1: Histone H4 (mono methyl R78) peptide. Lane 2: Histone H4 (asymmetric di methyl R78) peptide. Lane 3: Histone H4 (symmetric di methyl R78) peptide. Lane 4: Histone H4 unmodified peptide. Lane 5: Histone H4 (mono methyl K77) peptide. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody. Exposure time: 3 minutes. Blocking and dilution buffer: 5% NFDM/TBST.
  • Immunocytochemistry - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (ab251356)
    Immunocytochemistry - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (ab251356)
    This data was developed using ab202036, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 2% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H4 (mono methyl R78) with ab202036 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on Hela cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
  • Flow Cytometry - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (ab251356)
    Flow Cytometry - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (ab251356)
    This data was developed using ab202036, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Histone H4 (mono methyl R78) with ab202036 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
  • Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (ab251356)
    Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (ab251356)

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