Anti-Cytokeratin 4 antibody [EPR1598] (ab92465)
![Anti-Cytokeratin 4 antibody [EPR1598] (ab92465)](https://www.abcam.com/ps/products/92/ab92465/Images/ab92465-107474-anti-cytokeratin-4-antibody-epr1598-immunohistochemistry.jpg "Anti-Cytokeratin 4 antibody [EPR1598] (ab92465)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1598] to Cytokeratin 4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Cytokeratin 4 antibody [EPR1598]
See all Cytokeratin 4 primary antibodies -
Description
Rabbit monoclonal [EPR1598] to Cytokeratin 4 -
Host species
Rabbit -
Specificity
The immunogen shares high homology with other cytokeratins like cytokeratin-3(93%), cytokeratin-79(85.7%) and cytokeratin-2 oral(85.7%) etc. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Cytokeratin 4.
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Positive control
- WB: A431 and Human uterus cell lysates IHC-P: Human tonsil tissue ICC/IF: A431 cells
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1598 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 4 antibody [EPR1598] (ab92465)
ab92465, at a 1/100 dilution, staining Cytokeratin 4 in paraffin embedded Human tonsil tissue by Immunohistochemistry. Note positive staining of squamous cells.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-Cytokeratin 4 antibody [EPR1598] (ab92465)](https://www.abcam.com/ps/products/92/ab92465/Images/ab92465-107475-anti-cytokeratin-4-antibody-epr1598-western-blot.jpg)
Western blot - Anti-Cytokeratin 4 antibody [EPR1598] (ab92465)All lanes : Anti-Cytokeratin 4 antibody [EPR1598] (ab92465) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : Human uterus cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 57 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 4 antibody [EPR1598] (ab92465)](https://www.abcam.com/ps/products/92/ab92465/Images/ab92465-107473-anti-cytokeratin-4-antibody-epr1598-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 4 antibody [EPR1598] (ab92465)Immunofluorescent staining of A431 cells, using ab92465 at a 1/100 dilution. -
![Anti-Cytokeratin 4 antibody [EPR1598] (ab92465)](https://www.abcam.com/ps/products/92/ab92465/Images/ab92465-4-benefits-of-recombinant-antibodies.png)
Anti-Cytokeratin 4 antibody [EPR1598] (ab92465)
