Anti-Wnt7a antibody (ab100792)
Key features and details
- Rabbit polyclonal to Wnt7a
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Wnt7a antibody
See all Wnt7a primary antibodies -
Description
Rabbit polyclonal to Wnt7a -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken, Chimpanzee, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human Wnt7a aa 250 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab113305) -
Positive control
- Recombinant human Wnt7a protein (ab116171) can be used as positive control in WB. In Western Blot, ab100792 gave a positive signal in Human kidney tissue lysate. This antibody gave a positive result in ICC in HepG2 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab100792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 39 kDa). Abcam recommends using 5% BSA as the blocking agent.
ICC/IF Use a concentration of 5 µg/ml. Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts. -
Tissue specificity
Expression is restricted to placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain. -
Involvement in disease
Defects in WNT7A are the cause of limb/pelvis-hypoplasia/aplasia syndrome (LPHAS) [MIM:276820]; also known as absence of ulna and fibula with severe limb deficiency. LPHAS is a limb-malformation disorder characterized by various degrees of limb aplasia/hypoplasia and joint dysplasia.
Defects in WNT7A are a cause of Fuhrmann syndrome (FUHRS) [MIM:228930]; also known as fibular aplasia or hypoplasia femoral bowing and poly- syn- and oligodactyly. Fuhrmann syndrome is a distinct limb-malformation disorder characterized also by various degrees of limb aplasia/hypoplasia and joint dysplasia. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 749914 Chimpanzee
- Entrez Gene: 101146237 Gorilla
- Entrez Gene: 7476 Human
- Entrez Gene: 22421 Mouse
- Omim: 601570 Human
- SwissProt: Q1KYL1 Chimpanzee
- SwissProt: Q1KYK7 Gorilla
- SwissProt: O00755 Human
see all -
Alternative names
- Protein Wnt-7a antibody
- Protein Wnt-7a precursor antibody
- Proto oncogene Wnt7a protein antibody
see all
Images
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Western blot - Anti-Wnt7a antibody (ab100792)Anti-Wnt7a antibody (ab100792) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 28 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Wnt7a antibody (ab100792)ICC/IF image of ab100792 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100792, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HepG2 and MCF7 cells at 5µg/ml.
Protocols
Datasheets and documents
References (17)
ab100792 has been referenced in 17 publications.
- Zhang R et al. lncRNA BCAR4 sponges miR-370-3p to promote bladder cancer progression via Wnt signaling. Int J Mol Med 45:578-588 (2020). PubMed: 31894304
- Wang L et al. miR-127 suppresses gastric cancer cell migration and invasion via targeting Wnt7a. Oncol Lett 17:3219-3226 (2019). PubMed: 30867752
- Vallon M et al. A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific Regulation of Wnt Bioavailability. Cell Rep 25:339-349.e9 (2018). PubMed: 30304675
- Huang X et al. Wnt7a activates canonical Wnt signaling, promotes bladder cancer cell invasion, and is suppressed by miR-370-3p. J Biol Chem 293:6693-6706 (2018). PubMed: 29549123
- Griveau A et al. A Glial Signature and Wnt7 Signaling Regulate Glioma-Vascular Interactions and Tumor Microenvironment. Cancer Cell 33:874-889.e7 (2018). PubMed: 29681511
Images
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Western blot - Anti-Wnt7a antibody (ab100792)Anti-Wnt7a antibody (ab100792) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 28 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-Wnt7a antibody (ab100792)ICC/IF image of ab100792 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100792, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HepG2 and MCF7 cells at 5µg/ml.
