Anti-Wnt7a antibody (ab183653)
Key features and details
- Rabbit polyclonal to Wnt7a
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Wnt7a antibody
See all Wnt7a primary antibodies -
Description
Rabbit polyclonal to Wnt7a -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human Wnt7a aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O00755 -
Positive control
- WB: Wnt7a transfected HEK-293T lysate. IHC-P: Mouse pancreas and stomach tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt7a antibody (ab183653)
Paraffin-embedded mouse pancreas tissue stained for secreted WNT7A protein using ab183653 at 1/500 in immunohistochemical analysis.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt7a antibody (ab183653)Paraffin-embedded mouse stomach tissue stained for secreted WNT7A protein using ab183653 at 1/500 in immunohistochemical analysis.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Western blot - Anti-Wnt7a antibody (ab183653)All lanes : Anti-Wnt7a antibody (ab183653) at 1/5000 dilution
Lane 1 : Non-transfected (–) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : Transfected (+) HEK-293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt7a antibody (ab183653) Image from Kijong and Kyueng-Whan et al., J Breast Cancer., 20(4):361-367. Fig 1.; doi:10.4048/jbc.2017.20.4.361. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.Kijong Yi, Kyueng-Whan Min, et al used ab183653 at 1/50 dilution in immunohistochemical analysis.
Representative photographs of Wnt7a expression in the cytoplasm of neoplastic cells (from breast cancer patients diagnosed with invasive ductal carcinoma).
(A) Negative, (B) Positive for Wnt7a. Original magnification ×200.
This study, along with the waiver of informed consent, was approved by the Institutional Review Board at Hanyang University Hospital (HYUH 2016-05-009).
Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.
